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slrR [2017-07-24 09:39:53]
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slrR [2017-07-24 09:39:53]

transcriptional activator (Xre family) of competence development and sporulation genes, represses SigD-dependent flagellar genes, antagonist of SlrA and SinR, has LexA-like autocleavage activity
locus
BSU34380
pI
9.63
mw
17.43 kDa
protein length
152 aa Sequence Blast
gene length
456 bp Sequence Blast
function
regulation of initiation of biofilm formation and of autolysis
product
transcriptional regulator (Xre family), SlrA antagonist
essential
no
synonyms
yveJ, slr
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.6|Transition state regulators]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.4|Regulation]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.4|Swarming]

    • Gene

    Coordinates
    3,530,101 → 3,530,559

    Phenotypes of a mutant

  • smooth colonies on MsGG medium, no [SW|biofilm formation] [Pubmed|22113911]

    • The protein

    Catalyzed reaction/ biological activity

  • [protein|920F91E748EE079FF864011D9052B073567C41E4|SlrR] binds to and inhibits the activity of [protein|C17C296F3EB2E106C380E3B5D784F3FA63F4C9B7|SlrA], [protein|C17C296F3EB2E106C380E3B5D784F3FA63F4C9B7|SlrA] indirectly stimulates the synthesis of [protein|920F91E748EE079FF864011D9052B073567C41E4|SlrR] by interacting with [protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR]. [protein|920F91E748EE079FF864011D9052B073567C41E4|SlrR] can bind to [protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR] and [protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR] directly represses the transcription of [protein|920F91E748EE079FF864011D9052B073567C41E4|SlrR]. [protein|920F91E748EE079FF864011D9052B073567C41E4|SlrR] indirectly derepresses its own gene. The heterocomplex of [protein|920F91E748EE079FF864011D9052B073567C41E4|SlrR]-[protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR] is a repressor of autolysin and motility genes and inhibits the repressor function of [protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR]. [Pubmed|19788541,20923420,20351052]
  • repression of transcription of ''[gene|497C9654DC7514FF738D18E1F083299C43003A4C|lytA]-[gene|E1F9922DF7273040F84E5AC207A9F4CAFB85DC75|lytB]-[gene|6A21293823151C6980BF52B31A4B249A8440F2E1|lytC]'' and ''[gene|E8B88CBE4F9121DFEEF099D7947CD1E1AE656160|lytF]'' [Pubmed|20351052]
  • autocleavage [Pubmed|20923420]

    • Protein family

  • [SW|Xre family]

    • Paralogous protein(s)

  • [protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR]

    • Modification

  • subject to self-cleavage via a LexA-like autopeptidase, this involves [protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|ClpC] and requires [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU] [Pubmed|26819068,20923420]

    • Effectors of protein activity

  • interaction with [protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR] triggers binding of SlrR to the promoters of ''[gene|497C9654DC7514FF738D18E1F083299C43003A4C|lytA]-[gene|E1F9922DF7273040F84E5AC207A9F4CAFB85DC75|lytB]-[gene|6A21293823151C6980BF52B31A4B249A8440F2E1|lytC]'' and ''[gene|E8B88CBE4F9121DFEEF099D7947CD1E1AE656160|lytF]'', resulting in their repression [Pubmed|20351052]

    • Expression and Regulation

    Operons

    genes
    [gene|920F91E748EE079FF864011D9052B073567C41E4|slrR]-[gene|7FCB9037FDDC176FCF76088ADE7387A6A37068A9|pnbA]
    description
    [Pubmed|21815947]

    regulatory mechanism

  • [protein|5872812AB61E92E2944E926915EB7FEE71BFA6D5|Abh]: activation, [Pubmed|19767430], in [regulon|5872812AB61E92E2944E926915EB7FEE71BFA6D5|Abh regulon]
  • [protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR]: repression, [Pubmed|19788541], in [regulon|5A6FBAE6553343092862CB79E150F934978C32A9|SinR regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

    • additional information

  • induction by sequestration of [protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR] by [protein|24D6A7DDCB25B99FBB670E9203F826596916B35C|SinI], [protein|C17C296F3EB2E106C380E3B5D784F3FA63F4C9B7|SlrA] [PubMed|15661000,19788541] or by SlrR itself [PubMed|20351052]
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    Biological materials

    Mutant

  • MGNA-A089 (slr::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/89 NBRP B. subtilis, Japan]
  • GP955 (''slrR''-''[gene|7FCB9037FDDC176FCF76088ADE7387A6A37068A9|pnbA]''::''cat''), available in [SW|Jörg Stülke]'s lab

    • Expression vector

  • pGP2905: expression in ''E. coli'', with N-terminal His-tag, in [SW|pWH844], available in [SW|Jörg Stülke]' lab

    • two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in [SW|Jörg Stülke]' lab

    • References

    Reviews

  • 20541494,23353768,24988880

    • Original publications

  • 23378512,23430750,21856853,22329926,22113911,20817675,18430133,18647168,19767430,19788541,20351052,20923420,24256735,26819068